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KMID : 0359320010410030381
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Korean Journal of Veterinary Research
2001 Volume.41 No. 3 p.381 ~ p.385
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Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block
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김재훈/Kim, Jae Hoon
김진현/김대용/황우석/윤희정/이중근/이병천/Kim, Jin Hyun/Kim, Dae Yong/Hwang, Woo Suk/Youn,, Hee Jeong/Lee, Jung Keun/Lee, Byung Chun
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Abstract
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Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inlfammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less senstive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.
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